Prof. Dr. Vildan N. Civelek: pH change inside the lipid vesicles by Fatty-Acids

Published 6 months ago

Vildan N. Civelek, M.D., Ph.D., Prof., Endocrinologist, E-mail: vncivelek@gmail.com, vncivelek@aol.com: A medical research study/video directed, created, copyrighted/owned and published by Prof. Dr. Vildan N. Civelek. In this medical research pH... more

Vildan N. Civelek, M.D., Ph.D., Prof., Endocrinologist, E-mail: vncivelek@gmail.com, vncivelek@aol.com: A medical research study/video directed, created, copyrighted/owned and published by Prof. Dr. Vildan N. Civelek. In this medical research pH level inside the phospholipid vesicles is studied. The vesicles are composed of phospholipids without any membrane protein. Addition of Fatty Acids (i.e., Palmitic Acid) changed the pH inside of vesicles that can be explained by flip-flop of un-ionized Fatty Acids from outside into the vesicles by simple diffusion. The real time recording is the time course of Fatty Acid induced pH change inside the phospholipid vesicles. Inside of vesicles is filled with an aqueous buffer that allows fatty-acid carboxyl(COOH) group to ionize and release Hydrogen ion [H+]. The acidification inside the vesicle is measured by fluorescence spectrofluorometric methods. Boston University, Department of Medicine Endocrinology/Diabetes&Metabolism, Boston, USA. less

Tags: research biochemistry medicine fatty-acid diffusion phospholipid endocrinology metabolism molecular biophysics educational